Derivation of conditionally immortalised mouse DRG cell lines

Rodents are used in academic and pharmaceutical research to obtain cells for culture and tissues for histology. Neuroscience research is particularly dependent on primary cultures of neurons prepared from rodents (mainly rats but increasingly from transgenic mice as well). This is because neurons do not divide and thus do not exist as cell lines. Pain is an important warning system to guard against tissue damage. Pathological pain, however, has no warning value and has huge economical, social and personal costs on society. “Pain” scientists study sensory neurons of the dorsal root ganglia (DRG) in order to delineate pain signalling and thus discover new analgesic drug targets. DRG neurons are a heterogeneous population of neurons with distinct functional and histochemical properties. However, published DRG-derived cell lines do not represent a particular type of DRG neurons but rather provide a “generic” sensory neuron environment. They do not represent all functional types of DRG neurons and thus do not replace the need for primary cultures to study other sensory neuron types. The aim of this proposal is to generate conditionally immortalised neuronal cell lines from adult mouse DRG neurons. We aim to isolate and functionally characterise enough clones to reflect the functional diversity of DRG neurons. We plan to use the established H2Kb-ts-A58 Immorto mouse which has been used by many groups to derive conditionally immortalised cell lines from various tissues. However, one drawback of the Immorto mouse is that all cells express the Immorto transgene and therefore a DRG culture from it will contain immortalised cells from neuronal, glial and non-neuronal origins we have devised a novel strategy in order to select against the survival and propagation of non-neuronal cell types from Immorto DRG cultures. The strategy is based on expressing the bifunctional reporter gene βgeo selectively in neuronal cells of the DRG using Cre recombinase. This will enable us to use antibiotic selection to purify clones of neuronal origin only. We believe that the impact on the 3Rs will be considerable and easy to realise. The generated cell line can be deposited and be made available for academic researchers to validate and use in their research. We will also make them available for drug companies to replace acute cultures for in vitro screening and testing experiments.

Doran C, Chetrit J, Holley MC, Grundy D, Nassar MA (2015) Mouse DRG Cell Line with Properties of Nociceptors. PLoS ONE 10(6): e0128670 doi:10.1371/journal.pone.0128670

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Project grant

Status:

Closed

Principal investigator

Dr Mohammed Nassar

Institution

University of Sheffield

Co-Investigator

Professor David Grundy
Professor Mathew Holley

Grant reference number

G1000000/1

Award date:

Nov 2010 - Aug 2014

Grant amount

£387,378