Schedule 1 stunning followed by decapitation

Please read the general principles of blood sampling page before attempting any blood sampling procedure.

Technique

Although suitable for all strains, this technique should only be used in rare circumstances and where there is exceptional scientific justification. The primary reason for using this technique is to obtain a large volume of blood that has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary, but it should be noted there is a risk of contamination from other body fluids and tissues.

In order to be deemed a Schedule 1 method of euthanasia, mice which have been stunned must be determined as dead before decapitation (e.g. via confirmation of cessation of circulation or exsanguination - see Section 1(4) of the amended ASPA). This method should only be carried out by people competent in this method for the species and size of the animal. Training for stunning and decapitation should be undertaken on dead animals.

Summary

Number of samples One
Sample volume Up to 1 ml
Equipment Suitable sharp instrument to decapitate, (e.g., guillotine or sharp scissors).
Staff resource One person is required to take the blood sample.
Other A high level of expertise is required for this technique.

Schedule 1 stunning followed by decapitation technique in other animals

Click here for information on schedule 1 stunning followed by decapitation for blood sampling in the mouse Click here for information on schedule 1 stunning followed by decapitation for blood sampling in the mouse
All blood sampling techniques in the mouse

Click here for information on blood vessel cannulation in the mouse Click here for information on tail snip blood sampling in the mouse Click here for information on tail vessel microsampling in the mouse Click here for information on tail vein blood sampling in the mouse Click here for information on saphenous vein blood sampling in the mouse Click here for information on retro-orbital blood sampling techniques in the mouse Click here for information on abdominal/thoracic blood vessel blood sampling in the mouse Click here for information on cardiac puncture blood sampling in the mouse  Click here for information on decapitation blood sampling techniques in the mouse