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NC3Rs: National Centre for the Replacement Refinement & Reduction of Animals in Research

Announcing five awards supporting 3Rs knowledge and skills exchange

30 April 2020
Two laboratory technicians, one of them is using a microscope

We are pleased to announce five new Skills and Knowledge Transfer awards, bringing the total awards funded to 20 since the scheme launched in 2017.

Each project promotes the exchange and uptake of models, tools or technologies with immediate and deliverable 3Rs impacts between research groups. By funding projects such as these we aim to de-risk the uptake of new models and provide opportunities to apply the 3Rs, both in the UK and globally, across medical, biological and veterinary research.

The Skills and Knowledge Transfer grants are an annual competition with awards of up to £75k available for up to 24 months. Applications made to the scheme include both a ‘developer’ and ‘end-user(s)’ of the model, tool or technology; either party can be the primary applicant.

This year’s awards are summarised below:

Dr Gyorgy Fejer, University of Plymouth, £75,000 – Establishment of novel macrophage cell lines to study the pathogenesis of respiratory bacterial pathogens in lung alveolar macrophages

With NC3Rs funding, Gyorgy has previously characterised a continuously growing, non-transformed primary lung alveolar macrophage cell line replacing organ-derived or ex vivo produced primary macrophages. The 3Rs potential of the publication first describing these cells was also highly commended in the International 3Rs Prize.

Gyorgy will now train scientists from King’s College London and the Institut Pasteur, Korea to establish new macrophage cell lines from genetically modified animals. These cell lines will be used in pathogenesis studies with respiratory bacteria replacing up to 100 mice required annually in each laboratory.  

A graphic of a lung ultramarine

Dr Alasdair Nisbet, Moredun Research Institute, £20,598 – Using a novel, high welfare in vivo feeding device for poultry red mite and its integration with other novel tools for this parasite

Alasdair will host a workshop to train researchers from six academic institutions, both in the UK and overseas, in the use of an ‘on-hen’ feeding device. The NC3Rs-funded device allows precise numbers of red mites to feed on hens for up to three hours, avoiding the need for large field trials where birds are exposed to mites continuously for up to 30 weeks.

Using the ‘on-hen feeding’ device also reduces the number of hens needed for studies by 99%, from approximately 800 to eight animals for a vaccine study.

A graphic of a chicken ultramartine

Dr Ben Davies, University of Oxford, £71,242 – Naturally sterile hybrid mice for the production of embryo transfer recipients

Breeding programmes for genetically modified animals require modified embryos to be transferred into recipient female mice. Recipient mice are mated with sterile males in preparation for receiving the embryo, meaning male mice either undergo surgical vasectomy under anaesthesia or require extensive breeding programmes to generate sterility. Ben has established a new method by breeding hybrid male mice, which results in sterility in all male animals (compared to 12.5% in genetically modified breeding programmes) reducing the number of animals required.

Collaborating with MRC Harwell and the University of Manchester, Ben will be validating the pseudopregnancy rate of recipient mice mated with hybrid sterile males against historical data from all three facilities. Hybrid embryos will be cryopreserved for use by other researchers.

A graphic of a sperm and egg ultramartine

Dr Paul Dodson and Dr Riccardo Avvisati, University of Bristol, £73,588 - WaterR: A tool for better management and monitoring of rodent fluid intake

Paul and Riccardo will transfer their WaterR system to four UK research institutes where fluid reward is used as a motivator in behavioural experiments with mice. Such studies require the animals’ access to fluid to be restricted and the WaterR system enables automation of fluid control and tailoring of fluid restriction to each mouse within a group-housed cage preventing dehydration. It is comprised of readily available parts and each unit costs less than £50 to set up.

Paul and Riccardo will use this award to adapt WaterR to be compatible with different animal house set-ups enabling wider uptake.

A graphic of a mouse ultramartine

Dr Stuart Peirson, University of Oxford, £35,120 – Sharing new non-invasive circadian phenotyping methods

Stuart has developed non-invasive methods to measure sleep and body temperature rhythms in mice, aspects of behaviour and physiology that are regulated by circadian rhythms. These methods use infrared and thermal imaging and provide refinements to circadian rhythms studies, which usually require surgical implantation of electrodes under anaesthesia.

Stuart will host a series of hands-on workshops in Oxford to train researchers from Berlin in using the new methods and analysing the datasets generated.

Cancer Research UK requested that we suspend our agreement to make joint awards under the Skills and Knowledge Transfer scheme for the 2020 competition in light of the current situation and the impact it is having on their activities. For further details on the impact of COVID-19 on CRUK’s research community, please see their open letter.