Skip to main content
NC3Rs: National Centre for the Replacement Refinement & Reduction of Animals in Research
Pilot study grant

Refining the mouse model of footpad immunisation and infection

A pink eppendorf rack

At a glance

Award date
December 2014 - February 2016
Grant amount
Principal investigator
Dr Owain Millington
University of Strathclyde


  • Refinement
Read the abstract
View the grant profile on GtR

Application abstract

Our aim is to refine the 'traditional' model of footpad immunisation and infection in order to improve animal welfare. We will use a combination of immunological and cutting-edge imaging methods to define the key parameters associated with the administration of antigens, cells or parasites in the dorsal aspect to the foot, comparing it to the established footpad model. We will also assess development of Leishmania infection at this alternative site to establish whether parasite growth and ease of monitoring lesion development are comparable. Critically, we will use a combination of methods to assess any improvement in animal welfare in order to determine whether our proposal truly represents a refinement to currently used methods.