Skip to main content
NC3Rs: National Centre for the Replacement Refinement & Reduction of Animals in Research
Project grant

In vitro, off-host, rearing of the poultry red mite to supply parasites for development of new control methods

Test tubes

At a glance

In progress
Award date
March 2021 - February 2024
Grant amount
£463,088
Principal investigator
Dr Alasdair Nisbet
Institute
Moredun Research Institute

R

  • Replacement
Read the abstract
View the grant profile on GtR

Application abstract

Infestation of hen houses with poultry red mites (Dermanyssus gallinae) is a major animal welfare and economic problem for the egg-producing industry internationally and multiple groups worldwide are engaged in the development of new control methods for this parasite. Because D. gallinae is an obligate blood-feeding parasite, to supply mites for any research programme, donor hens must be infested with the parasite to provide sufficient numbers of mites for subsequent laboratory or field studies. The aim of the current proposal is to replace the use of mite-infested hens as a source of parasite material by establishing a laboratory colony of mites, using a novel in vitro feeding methodology designed in our group, which is both sustainable for the routine culturing of mites and scalable for the production of the large numbers required for trials (e.g. vaccine studies or the development of novel acaricides) when required. Through a series of preliminary experiments in our laboratory, we have established that poultry red mites will feed, off-host, in an in vitro feeding device containing goose blood which is derived from our donor flock of geese. Geese are particularly suited to be blood donors for this task as they can supply a much greater volume of blood per procedure (bleed) than hens (approximately 30-fold). By following a series of 3 Objectives we will optimize this system for routine mite culturing and scaling up for large numbers of mites required for larger studies:
Objective 1 will establish optimal mite feeding protocols to maintain mite colonies long term through an analysis of multiple nutritional and environmental parameters;
Objective 2 will determine if lab-reared mites behave in the same way as field-caught mites when exposed to a host;
Objective 3 will optimise mite housing and feeding for sustainable expansion of colony numbers.

Impacts