Mouse handling research papers

Below are links to the original papers which provide the evidence-base for the non-aversive handling methods, as well as papers which validate or use the refined handling techniques.

The original papers:

  • Hurst JL, West RS (2010) Taming anxiety in laboratory mice. Nature Methods 7:825-826. doi:10.1038/nmeth.1500
  • Gouveia K, Hurst JL (2013) Reducing mouse anxiety during handling: Effect of experience with handling tunnels. PLoS ONE 8(6): e66401. doi:10.1371/journal.pone.0066401 
  • Gouveia K, Hurst JL (2017) Optimising reliability of mouse performance in behavioural testing: the major role of non-aversive handling. Scientific Reports 7: 44999. doi:10.1038/srep44999

Papers that validate the improved handling techniques:

  • Ghosal S et al. (2015) Mouse handling limits the impact of stress on metabolic endpoints. Physiology and Behaviour 150:31-37. doi:10.1016/j.physbeh.2015.06.021
  • Clarkson JM, Dwyer DM, Flecknell PA, Leach MC, Rowe C (2017) Handling method alters the hedonic value of reward in laboratory mice. Scientific Reports 8: 2448. doi:10.1038/s41598-018-20716-3
  • Nakamura Y, Suzuki K (2018) Tunnel use facilitates handling of ICR mice and decreases experimental variation. J Vet Med Sci 80(6): 886-892. doi:10.1292/jvms.18-0044