- Resources and references
- Schedule 1 stunning followed by decapitation sampling in other animals
- All blood sampling techniques in the rat
Please read the general principles of blood sampling page before attempting any blood sampling procedure.
Although suitable for all rat strains, this technique should only be used in rare circumstances and where there is exceptional scientific justification. Further, the ASPA limits its use to rodents of body weight 1kg or less. The primary reason for using this technique is to obtain a large volume of blood that has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary, but it should be noted there is a risk of contamination from other body fluids and tissues.
In order to be deemed a Schedule 1 method of euthanasia, rats which have been stunned must be determined as dead before decapitation can take place (e.g. via confirmation of cessation of circulation or exsanguination - see Section 1(4) of the amended ASPA). This method should only be carried out by people competent in this method for the species and size of the animal. Training for stunning and decapitation should be undertaken on dead animals.
|Number of samples||One|
|Sample volume||Up to 10 ml|
|Equipment||Suitable sharp instrument to decapitate, (e.g., guillotine or sharp scissors).|
|Staff resource||One person|
|Other||A high level of expertise is required for this technique.|