Schedule 1 stunning followed by decapitation

Please read the general principles of blood sampling page before attempting any blood sampling procedure.

Technique

Although suitable for all rat strains, this technique should only be used in rare circumstances and where there is exceptional scientific justification. Further, the ASPA limits its use to rodents of body weight 1kg or less. The primary reason for using this technique is to obtain a large volume of blood that has not been affected by anaesthetic drugs or carbon dioxide. A large volume of blood can be collected from the trunk if necessary, but it should be noted there is a risk of contamination from other body fluids and tissues.

In order to be deemed a Schedule 1 method of euthanasia, rats which have been stunned must be determined as dead before decapitation can take place (e.g. via confirmation of cessation of circulation or exsanguination - see Section 1(4) of the amended ASPA). This method should only be carried out by people competent in this method for the species and size of the animal. Training for stunning and decapitation should be undertaken on dead animals.

Summary

Number of samples One
Sample volume Up to 10 ml
Equipment Suitable sharp instrument to decapitate, (e.g., guillotine or sharp scissors).
Staff resource One person
Other A high level of expertise is required for this technique.

Schedule 1 stunning followed by decapitation sampling in other animals

Click here for information on schedule 1 stunning followed by decapitation for blood sampling in the mouseClick here for information on schedule 1 stunning followed by decapitation for blood sampling in the guinea pig

All blood sampling techniques in the rat

Click here for information on blood vessel cannulation in the ratClick here for information on tail vein blood sampling in the ratClick here for information on jugular vein blood sampling in the ratClick here for information on saphenous vein blood sampling in the ratClick here for information on retro-orbital blood sampling techniques in the ratClick here for information on using a temporary cannula for blood sampling in the ratClick here for information on abdominal/thoracic blood vessel blood sampling in the ratClick here for information on cardiac puncture blood sampling in the ratClick here for information on decapitation blood sampling techniques in the rat