Using the Drosophila fly intestine to investigate Wnt targets in vivo

The Apc tumour suppressor gene is mutated in 80% of colorectal cancers. Mechanistically its major function as a tumour suppressor is to negatively regulate Wnt signalling, preventing the activation of Wnt target genes. Due to the difficulties of studying intestinal cells ex vivo, a plethora of studies have been performed investigating gene function within the murine intestine following Apc loss. In this grant we propose to validate the Drosophila fly intestine as a model system to investigate Apc loss. Specifically we aim to use the Drosophila intestine as a screen for Wnt targets that are functionally important for the phenotypes of Apc loss. These experiments could dramatically reduce the numbers of murine intestinal experiments performed and yield new insights in to colorectal carcinogenesis.

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Project grant

Status:

Closed

Principal investigator

Dr Owen Sansom

Institution

University of Glasgow

Co-Investigator

Dr Marcos Vidal

Grant reference number

G1000078/1

Award date:

Nov 2010 - Nov 2013

Grant amount

£350,528

Primary 'R'

Replacement

Scientific Discipline

Cells and systems
Cancer

Technologies/approach

Non-vertebrate models

Keywords

Fly
Genetics
Oncology