Decision tree

How much blood does a mouse have?

On average, mice have around 58.5 ml of blood per kg of bodyweight.

A mouse weighing 25 g would therefore have a total blood volume (TBV) of approximately 58.5 ml/kg x 0.025 kg = 1.46 ml.

How to decide on the most appropriate blood sampling technique for mice?

The two tables below are designed to assist in determining the amount of blood to sample from the animal, and depending on that volume, the most appropriate techniques to use.

1. Do you require more than one blood sample from the same mouse?

YES

NO

Maximum <10% TBV (= 0.14 ml) on any single occasion AND <15% TBV ( = 0.21 ml) in 28 days

Maximum <10% TBV ( = 0.14 ml)

For repeat bleeds at short intervals, suggested limit <1% TBV (= 0.01 ml) in 24 hours AND consider cannulation

OR terminal sample under general anaesthesia (volume unrestricted)

 

2. How much blood do you require?

Total of <0.20 ml

Total of >0.20 ml

General anaesthesia required

General anaesthesia not required

General anaesthesia required; non recovery

Saphenous vein

Tail vein

Sublingual vein*

Saphenous vein

Tail vein

Mandibular vein**

Blood vessel cannulation***

Tail snip ****

Cardiac puncture

Abdominal / thoracic blood vessel

Retro-orbital

Decapitation #

* Not widely used (Hiemann et al. 2009)
** Not widely used (Golde et al. 2005)
*** Cannulation surgery performed under general anaesthesia.
**** Not recommended as more refined methods of sampling exist. Plus a mixed arterial / venous sample is obtained.
# Blood may be mixed with tissue fluid.