Refinement of rearing practices in marmosets

The project will investigate how to refine breeding and rearing to promote best practice for welfare and research in the common marmoset (Callithrix jacchus). This small monkey is the most frequently used New World primate in European laboratories, primarily for toxicology. There are considerable welfare problems associated with breeding marmosets, including high infant mortality from large litter sizes (twins are the norm in the wild), birth complications, and rearing practices that often necessitate early deprivation from the family which is known to have adverse effects on behaviour and physiology. Studies will be conducted at a breeding facility which supplies marmosets to laboratories. Carefully designed experiments will compare infant development, family interactions, response to routine stressors, weight gain, learning and motivation in marmosets born to litters of different sizes and reared in different conditions. These conditions include (a) marmoset-reared twins (control) (b) marmoset-reared infants from triplets (c) rotationally-reared triplets (d) foster-reared triplets (e) human-reared infants from triplets and (f) marmoset-reared singletons. The findings, combined with back record analyses will determine factors affecting litter size, dam mortality and best rearing practices for welfare when litters exceed two. Should, as anticipated, the results demonstrate that marmoset-reared twins have the best welfare, the lowest mortality, fewest birth complications and produce the best models, then evidenced-based recommendations to husbandry to promote twin births will be trialled. As heavier dams produce larger litters, this may include maintaining dams at weights similar to wild counterparts to increase the incidence of twins births. When litters exceed two, another refinement may be to train marmosets to allow care staff to provide supplementary food for carried infants to obviate separation. The findings should have far-reaching implications for quality of life of marmosets in breeding facilities, and for the reliability of the marmoset as a model in research and testing.

Ash H et al. (2021). The long-term impact of infant rearing background on the behavioural and physiological stress response of adult common marmosets (Callithrix jacchus). Applied Animal Behaviour Science 234:105169. doi: 10.1016/j.applanim.2020.105169

Ash H et al. (2018). Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique. Physiology and Behavior 185:14-22. doi: 10.1016/j.physbeh.2017 

Ash H and Buchanan-Smith HM (2016). The long-term impact of infant rearing background on the affective state of adult common marmosets (Callithrix jacchus). Applied Animal Behaviour Science 174:128-136. doi: 10.1016/j.applanim.2015.10.009

Ash H and Buchanan-Smith HM (2014). Long-term data on reproductive output and longevity in captive female common marmosets (Callithrix jacchus). American Journal of Primatology 11:1062-1073. doi: 10.1002/ajp.22293

• Blog: Reflections of an NC3Rs student
• Further Funding: NC3Rs Project Grant, Validating reward-related behaviour for welfare assessment, and improving welfare through increased predictability of events, April 2013, £168,345